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·1586· 精细化工 FINE CHEMICALS 第 37 卷

2.8 Tyr/Hf-ZnO/CS/GCE 的选择性和抗干扰性研究 sensitive phenol and atrazine sensing[J]. Talanta, 2016, 160: 125-132.
[3] ZHOU Y Y, TANG L, ZENG G M, et al. Mesoporous carbon nitride
分别选用多巴胺、抗坏血酸、葡萄糖、尿素等
based biosensor for highly sensitive and selective analysis of
与邻苯二酚电化学活性相近且可能影响酪氨酸酶催 phenol and catechol in compost bioremediation[J]. Biosensors and
化活性的物质进行抗干扰实验，Tyr/Hf-ZnO/CS/GCE Bioelectronics, 2014, 61: 519-525.
[4] LUO X L, ZHENG H, ZHANG Z H, et al. Cloud point extraction for
的抗干扰实验结果见图 11。可以看出，当加入邻苯 simultaneous determination of 12 phenolic compounds by high
二酚溶液时，该工作电极出现明显的电流响应，但 performance liquid chromatography with fluorescence detection[J].
是加入相同浓度的其他底物时，除加入抗坏血酸时 Microchemical Journal, 2018, 137: 148-154.
[5] LI T T, JIA Q, SONG L H, et al. Coupling poly-(methacrylic acid-co-
有微弱响应外，其余均无响应，说明 Tyr/Hf-ZnO/CS/ ethylene glycol dimethacrylate) monolith microextraction to capillary
GCE 具有很好的抗干扰能力。 electrophoresis for the determination of phenols in water samples[J].
Talanta, 2009, 78, 4(5): 1497-1502.

[6] ZEHANI N, FORTGANG P, LACHGAR M, et al. Highly sensitive
electrochemical biosensor for bisphenol a detection based on a
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with a multi-walled carbon nanotube-tyrosinase hybrid film[J].
Biosensors and Bioelectronics, 2015, 74: 830-835.
[7] SEO S Y, SHARMA V K, SHARMA N. Mushroom tyrosinase:
Recent prospects[J]. Journal of Agricultural and Food Chemistry,
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selective magnetite (Fe 3O 4) and tyrosinase immobilized on chitosan
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gold nanoparticles as matix for tyrosinase for amperometric catechol
图 11 添加其他干扰物对 Tyr/Hf-ZnO/CS/GCE 响应邻苯 biosensor[J]. Sensors and Actuators B: Chemical, 2013, 182: 161-
二酚的影响 169.
Fig. 11 Amperometric response of Tyr/Hf-ZnO/CS/GCE [10] EREMIA S A V, VASILESCU I, RADOI A, et al. Disposable biosensor
biosensor towards the addition of different based on platinum nanoparticles-reduced graphene oxide-laccase
interfering substances biocomposite for the determination of total polyphenolic content[J].

Talanta, 2013, 110: 164-170.
3 结论 [11] LETE C, LUPU S, LAKARD B, et al. Multi-analyte determination
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通过水热法合成了 Hf-ZnO 材料，Hf-ZnO 纳米 Journal of Electroanalytical Chemistry, 2015, 744: 53-61.
材料的平均粒径为 80.5 nm，且颗粒分散均匀。将 [12] VICENTINI F C, GARCIA L L C, FIGUEIREDO-FILHO L C S, et al.
A biosensor based on gold nanoparticles, dihexadecylphosphate, and
Hf-ZnO、Tyr 和 CS 共同构建了 Tyr/Hf-ZnO/CS/GCE
tyrosinase for the determination of catechol in natural water[J].
工作电极，并在 K 3 Fe(CN) 6 /K 4 Fe(CN) 6 溶液中探究其 Enzyme and Microbial Technology, 2016, 84: 17-23.
电化学性能，其峰电流与扫描速率的平方根呈线性 [13] KARIMI K G, MOZAFFARI S A, EBRAHIMI M, et al. Spin-coated
ZnO-graphene nanostructure thin film as a promising matrix for urease
关系，电极反应是扩散控制过程。Tyr/Hf-ZnO/CS/ immobilization of impedimetric urea biosensor[J]. Journal of the
GCE 生物传感电极在 pH=5.0，工作电压–0.05 V 的 Chinese Chemical Society, 2018, 65(11): 1379-1388.
[14] GU B X (谷保祥), WANG X Y (王喜英), QIAO M X (乔明晓), et al.
条件下对邻苯二酚具有最佳的检测性能，而–0.05 V
The study of phenol pollution water detection based on nanostructure
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高的灵敏度，为 195 mA/(mol/L)。线性范围是 0.5~ properties in composition controlled Hf: ZnO thin films grown by
2
47.0 µmol/L（R =0.9938），检测限为 0.1215 µmol/L atomic layer deposition[J]. Journal of the Electrochemical Society,
2012, 159(4): 384-387.
（S/N=3）。此外，该生物电极表现出良好的稳定性
[16] YIN Q Q (殷巧巧), QIAO R (乔儒), TONG G X (童国秀), et al.
和重复性，其对邻苯二酚 5 次测试的标准偏差 RSD Preparation and photocatalytic application of ion-doped ZnO functional
仅为 4.05%。 nanomaterials[J]. Chemical Industry and Engineering Progress (化
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