Page 140 - 《精细化工》2022年第8期
P. 140

·1640·                            精细化工   FINE CHEMICALS                                 第 39 卷

            射流辅助提取对鱼鳞多糖对 DPPH•清除能力影响的                              production[J]. Journal of Applied Microbiology, 2010, 109(6): 2160-2172.
                                                               [4]   LAKSHMINARAYANAN R, KESSELMAN E, COHEN R, et al.
            结果一致。由图 7b 可知,EBGS 和 EBG 对•OH 具
                                                                   Curdlan complexes as a potential  food-grade delivery system:
            有一定的清除能力,而且清除能力与其质量浓度有                                 Genistein case study[J]. Ther Deliv, 2011, 2(2): 181-192.
            关,这与 DPPH•清除能力结果相似。当样品质量浓                          [5]   CLARKE  A E, STONE  B A. Structure of the paramylon from
                                                                   Euglena gracilis[J]. Biochim Biophys Acta, 1960, 44(1): 161-163.
            度在 0.5~1.0 g/L 时,随着质量浓度的增加,对•OH                    [6]   BARSANTI L, PASSARELLI V, EVANGELISTA V, et al. Chemistry,
            的清除能力迅速增大,而当质量浓度>1.0 g/L 时,                            physico-chemistry and applications linked to biological activities of
                                                                   β-glucans[J]. Natural Product Reports, 2011, 28: 457-466.
            对•OH 清除能力增加不明显。这可能与本文的样品                           [7]   LI X Q (栗晓庆), LYU J P (吕俊平), LIU Q (刘琪), et al. Optimization
            质量浓度较高有关,当样品质量浓度较高时,—CH                                of alkaline extraction and antioxidant activities of paramylon  in
                                                                   vitro[J]. Food Science and Technology (食品科技), 2019, 44(9):
            基团导致多糖的整合,并在溶液中产生簇,簇的存
                                                                   209-215.
            在减少了•OH 与多糖接触的机会              [34] ,从而导致•OH        [8]   JU H J (鞠海军).  Separation, structure identification of paramylon
            清除能力较低。综上所述,UHPM 提取得到的 EBG                             and study on its bioactivities[D]. Shanghai: East China University of
                                                                   Science and Technology (华东理工大学), 2020.
            对 DPPH•的清除能力有一定的增强,对•OH 清除能                        [9]   VISMARA R, VESTRI S, FRASSANITO A M , et al. Stress resistance
            力效果不明显,但都不及 V C 。                                      induced by paramylon treatment in Artemia sp.[J]. Journal of Applied
                                                                   Phycology, 2004, 16(1): 61-67.
            3   结论                                             [10]  KONDO Y, KATO A, HOJO  H,  et al. Cytokine-related
                                                                   immunopotentiating activities of paramylon, a  β-(1→3)-D-glucan
                                                                   from Euglena gracilis[J]. Journal of Pharmacobio-Dynamics, 1992,
                (1)通过单因素及响应面设计实验,优化了                               15(11): 617-621.
            UHPM 时间、压力、料液比及 SDS 质量浓度对 EBG                      [11]  BROWN G D, GORDON S. Fungal  β-glucans and mammalian
                                                                   immunity[J]. Immunity, 2003, 19(3): 311-315.
            提取率的影响。EBG 提取最佳条件为时间 93 s、压                        [12]  HUNTER J K W, GAULT  R A, BERNER M D. Preparation of
            力 34 MPa、料液比 1∶270(g∶mL)、SDS 质量浓                       microparticulate β-glucan from Saccharomyces cerevisiae for use in
                                                                   immune potentiation[J]. Letters in Applied Microbiology, 2002, 35:
            度 4.2 g/L。在此条件下,EBG 提取率为 80.27%。
                                                                   267-271.
            碱法提取 EBG 提取率仅为 42.35%,而且破坏了 EBG                    [13]  SUTHERLAND I W. Novel and established applications of microbial
            的三螺旋结构;超声波处理时间为 18.5 min 时,裸                           polysaccharides[J]. Trends in Biotechnology, 1998, 16: 41-46.
                                                               [14]  BROWN L, ROSNER  B, WILLETT W W,  et al. Cholesterol-
            藻多糖得率为 95.26%。与前两者相比本实验效率更                             lowering effects  of dietary fiber:  A meta-analysis[J]. American
            高、用时更少,节约了能源,保证了产品质量。                                  Journal of Clinical Nutrition, 1999, (1): 30-42.
                                                               [15]  TREPEL F. Dietary fibre: More than a matter of dietetics.  Ⅰ.
                (2)经过刚果红实验及 FTIR、XRD、SEM 分
                                                                   Compounds,  properties, physiological effects[J].  Wiener klinische
            析,超高压微射流技术提取的 EBG 仍具有有序的三                              Wochenschrift, 2004, 116(14): 465-476.
            螺旋结构;EBG 的结晶度降低,EBG 外表出现裂痕                         [16]  CHAN C F, CHAN W K, SZE M  Y. The effects of  β-glucan on
                                                                   human immune  and cancer  cells[J]. Journal of Hematology &
            与空隙。                                                   Oncology, 2009, 2(1): 1-11.
                (3)测定 EBGS 和 EBG 对 DPPH•和•OH 的清                [17]  ZHAO Q L, YAN W Q, LIU Y F, et al. Modulation of the structural
                                                                   and functional properties of perilla protein isolate from oilseed
            除能力,在质量浓度为 0.4~2.0 g/L 时 EBGS 和 EBG
                                                                   residues by dynamic high-pressure microfluidization[J]. Food Chemistry,
            对 DPPH•清除率基本相同,在质量浓度为 4.0 g/L                          2021, 365: 130497.
            时,EBG 对 DPPH•清除率达到 50.26%,高于 EBGS                  [18]  KASEMWONG K, RUKTANONCHAI U R, SRINUANCHAI W,
                                                                   et al. Effect of high-pressure microfluidization on the structure of
            对 DPPH•的清除率(35.14%)。而 EBGS 和 EBG 对                     cassava starch granule[J]. Starch, 2011, 63(3): 76-81.
            •OH 清除效果不明显,清除率相对较低。                               [19]  QIN L X (秦令祥), ZHOU J Q (周婧琦), CUI S W (崔胜文), et al.
                                                                   Study on extraction of polysauharides from Auricularia auricular by
                 EBG 的结构特点使其水溶性较差,抗氧化活性
                                                                   dynamic high pressure[J]. Food Research and Development (食品研
            较弱,限制了其在食品、医药等领域的应用。所以                                 究与开发), 2019, 40(19): 155-159.
            增强 EBG 的水溶性是亟待解决的问题。                               [20] WU  Y  (吴云), CHEN W  (陈薇),  TIAN  W H (田文慧),  et al.
                                                                   Ultrasonic extraction optimization of  raspberry (Rubus idaeus  L.)
            参考文献:                                                  pulp polysaccharides and its anti-fatigue activity[J]. Natural Product
                                                                   Research and Development (天然产物研究与开发), 2020, 32(12):
            [1]   IVANOVA I M, NEPOGODIEV S A, SAALBACH G, et al. Fluorescent   2081-2093.
                 mannosides serve as acceptor substrates for glycosyltransferase and   [21]  WANG Z M, CHEUNG Y C, LEUNG P H, et al. Ultrasonic treatment
                 sugar-1-phosphate transferase activities in Euglena gracilis membranes[J].   for  improved  solution properties of a high-molecular weight
                 Carbohydrate Research, 2017, 438: 26-28.          exopolysaccharide produced by a medicinal fungus[J]. Bioresource
            [2]   BARSANTI  L,  VISMARA R, PASSARELLI V,  et al. Paramylon   Technology, 2010, 101(14): 5517-5522.
                 (β-1,3-glucan) content in wild  type and WZSL mutant of  Euglena   [22]  WEN Y X (文愉熙), HUANG X Z (黄晓舟), LIN X  S (林晓思).
                 gracilis. Effects of growth conditions[J]. Journal of Applied Phycology,   Analysis of chemical composition and antioxidant activities of
                 2001, 13(1): 59-65.                               non-water-soluble and water-soluble polysaccharides from Euglena
            [3]   RODRÍGUEZ-ZAVALA J  S, ORTIZ-CRUZ M A, MENDOZA-   gracilis[J]. Science and Technology of Food Industry (食品工业科
                 HERNANDEZ G, et al. Increased synthesis of α-tocopherol, paramylon   技): 2022, 43(5): 105-113.
                 and tyrosine by Euglena gracilis under conditions of high biomass           (下转第 1661 页)
   135   136   137   138   139   140   141   142   143   144   145