Page 147 - 《精细化工》2022年第11期

第 39 卷第 11 期 精 细 化 工 Vol.39, No.11
2 022 年 11 月 FINE CHEMICALS Nov. 2022

生物工程
两种方法提取琉璃苣叶多糖的理化性质及生理活性

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张喜峰 1,2 ，南 艳 ，李雪茹 ，何立杰 ，刘 霞 1,2 ，罗光宏
（1. 河西学院 生命科学与工程学院，甘肃 张掖 734000；2. 甘肃省河西走廊特色资源利用重点实验室，
甘肃 张掖 734000；3. 河西学院 甘肃省微藻技术创新中心，甘肃 张掖 734000）

摘要：分别采用纤维素酶辅助和微波辅助两种方法提取琉璃苣叶多糖，得到的多糖分别记为 BLP-1 和 BLP-2。
采用高效凝胶渗透色谱仪、FTIR、SEM 及 TGA 对 BLP-1 和 BLP-2 进行了表征，通过 α-葡萄糖苷酶和 α-淀粉酶
的抑制效果评价样品的体外降血糖活性，通过检测对人肝癌细胞 HepG2、人乳腺癌细胞 MX-1 和人肺腺癌细胞
A549 的抑制率评价了样品的抗癌活性，通过检测小鼠巨噬细胞 RAW246.7 的免疫因子分泌评价样品免疫活性。
结果表明，BLP-1 和 BLP-2 具有不同的多糖和蛋白质质量分数。BLP-1 和 BLP-2 由 L-阿拉伯糖、D-半乳糖、葡
萄糖、木糖组成，但摩尔分数不同，其相对分子质量分别为 20103 和 22652。BLP-2 比 BLP-1 具有更稳定的结
构。这两种多糖具有抑制 α-葡萄糖苷酶和 α-淀粉酶活性、抑制上述 3 种癌细胞增殖以及激活小鼠巨噬细胞
RAW264.7 细胞分泌免疫细胞因子以介导细胞免疫反应的潜力。
关键词：琉璃苣；多糖；理化性质；生理活性；生物工程
中图分类号：R284.2；R285 文献标识码：A 文章编号：1003-5214 (2022) 11-2297-08

Physicochemical properties and physiological activities of polysaccharides
extracted from Borage leaves by two methods

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ZHANG Xifeng , NAN Yan , LI Xueru , HE Lijie , LIU Xia , LUO Guanghong
（1. School of Life Science and Engineering, Hexi University, Zhangye 734000, Gansu, China; 2. Key Laboratory of
Hexi Corridor Resources Utilization of Gansu, Zhangye 734000, Gansu, China; 3. Microalgae Technology Innovation
Center of Gansu Province, Hexi University, Zhangye 734000, Gansu, China）
Abstract: Borage leave polysaccharides，obtained by cellulose enzyme assisted extraction and microwave
assisted extraction, were named as BLP-1 and BLP-2, respectively, and then characterized by high
performance gel permeation chromatography, FTIR, SEM and TGA. Furthermore, the in vitro
hypoglycemic, anti-cancer and immune activities of BLP-1 and BLP-2 was evaluated by the inhibitory
effect of α-glucosidase and α-amylase, inhibition rate of HepG2 human hepatoma cells, MX-1 human breast
cancer cells and A549 human lung adenocarcinoma cells, and immune factor secretion detection in mouse
macrophages RAW246.7, respectively. The results showed that the sugar and protein contents in BLP-1 and
BLP-2 were different. Though both of BLP-1 and BLP-2 were composed of L-arabinose, D-galactose,
glucose, and xylose, their molar fraction was different with corresponding relative molecular mass of 20103
and 22652, respectively. Meanwhile, BLP-2 exhibited a more stable structure than BLP-1. The two
polysaccharides showed potential in inhibiting α-glucosidase and α-amylase activities, suppressing the
above cancer cells proliferation, and activating mouse macrophages RAW264.7 cells to secret immune
cytokines for cellular immune response mediation.
Key words: Borago officinalis L.; polysaccharides; physicochemical properties; physiological activities;
biological engineering

收稿日期：2022-01-25; 定用日期：2022-06-09; DOI: 10.13550/j.jxhg.20220107
基金项目：甘肃省科技计划项目（18JR2JG001）；甘肃省高等学校创新基金项目（2021B-222）；教育部产学合作协同育人项目
（202102462031）
作者简介：张喜峰（1982—），男，副教授，E-mail：curiouslysxsd@163.com。联系人：罗光宏（1965—），男，教授，E-mail：
luoguanghong@163.com。